01 / GENETIC VARIATION
How do individuals differ genetically?
Generate sequence information around individual microbes to study genomic diversity within a population.
All platformsStart with individual microbes.
X1000 · SINGLE-MICROBE RESEARCH
Start with individual microbes.
Explore variation within populations.
Microfluidic partitioning, reactions and barcode association create a workflow for single-microbe genomic research, adding an individual-level view of population heterogeneity.
Ask about X1000 studies
RESEARCH QUESTIONS
Individual variation and sequence provenance.
02 / SEQUENCE ASSIGNMENT
How can sequence information be linked to individuals?
Use partition and barcode information to investigate sequence origin and association.
03 / FUNCTIONAL LEADS
Which functional questions arise from these differences?
Use genomic differences to inform hypotheses for functional research and test them in independent experiments.
HOW IT WORKS
From reaction compartments
to sequence provenance.
Microfluidic droplets provide space for sample partitioning and independent reactions. Compatible lysis, amplification and barcoding steps link sequence data to its source partition, and quality control then assesses genomic information at the individual level.
Separation and partitioning
Design suspension preparation and partitioning around the target organisms and sample conditions.
Lysis and amplification
Assess organism compatibility with steps such as lysis and genome amplification.
Source association and QC
Combine barcodes, quality control and analytical methods to investigate sequence-to-individual associations.
Occupancy control, multiplet detection and amplification QC select reliable single-organism data.
WORKFLOW
A single-microbe genomic research workflow.
- 01
Prepare the sample
Assess the sample matrix and target organisms, then prepare a suitable suspension.
- 02
Partition and process genomes
Perform the relevant droplet processing, lysis and genome-amplification steps.
- 03
Barcode and build libraries
Connect fragmentation, barcode association and library preparation.
- 04
Sequence and analyze
After external sequencing, interpret the data alongside quality control, coverage and analytical methods.
SYSTEM & ASSAY
Instrument, assay chemistry and quality analysis.
Instrument and droplet handling
Organize the experiment around microfluidic partitioning and the relevant reaction stages.
Assay chemistry
Assess processing and amplification for the organisms of interest, recognizing that different biological targets require different treatment.
Quality and analysis
Consider usable sequence, coverage and origin assignment, with their limitations interpreted in the context of the study.
RVQC® System (standard)
Every X1000 includes the RVQC® Real-Time Visual Quality Control System, which uses real-time visual recognition to monitor key droplet states.
START A STUDY
Organisms and sample feasibility.
A discussion can begin with three things: where the sample comes from, which organisms are of interest and what question you want to answer. These guide assessment of sample processing, assay compatibility and analysis scope.
Individual microbes and communitiesDesigned for single-microbe research such as bacteria; viral particles and infected host cells can be assessed per project.
Assess microbial project feasibilityFeedback, suggestions or concerns: CEO Direct