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R100 · SINGLE-CELL RNA & CHROMATIN RESEARCH

Understand cell composition.
Explore differences in state.

Start with single-cell transcriptomic information to study composition, expression and state in tissues or cell populations. Select an RNA or chromatin workflow around the question at hand.

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R100 platform image
R100 platform image

RESEARCH QUESTIONS

Cell composition, expression states and accessibility.

01 / CELL COMPOSITION

Which cell populations are present?

Use expression features and reference information to study population composition and relative differences.

02 / EXPRESSION STATE

Which expression programs change?

Compare cellular states and associated molecular signals across conditions within the limits of the study design.

03 / CHROMATIN ACCESSIBILITY

How can regulatory information add context?

Select an appropriate workflow, such as ATAC, to investigate chromatin accessibility.

SINGLE-CELL TRANSCRIPTOMIC PRINCIPLE

Preserve the cellular origin
of molecular signals.

Microfluidic droplets, barcoded beads and assay reactions carry cellular-origin and molecular-label information into library preparation and downstream analysis.

mRNA capture

In the relevant transcriptomic workflow, Poly(dT) captures transcripts with poly(A) tails.

Cell barcode

Retain cellular-origin information for downstream read assignment.

Unique molecular identifier (UMI)

Use molecular labels to help identify amplification duplicates and support molecular counting.

This section describes the transcriptomic workflow; ATAC uses a different experimental and analytical process.

FROM SAMPLE TO DATA

Single-cell transcriptomic workflow

  1. 01

    Assess and prepare samples

    Define the study groups and suitable preparation method, then prepare the required single-cell suspension.

  2. 02

    Capture and label

    Combine the instrument, capture components and barcoded beads for cell partitioning and assay reactions.

  3. 03

    Reverse transcribe and build libraries

    Use the reverse-transcription, library-preparation and index components to prepare transcriptomic libraries.

  4. 04

    Sequence and analyze

    After external sequencing, perform quality control, expression-data processing and the agreed research analyses.

SYSTEM COMPONENTS

Components for transcriptomic experiments.

Capture and barcoded beads

Cell-capture components and barcoded beads connect partitioning, capture and cellular labeling.

Reverse transcription

Reverse-transcription reagents transfer captured transcript information into the library workflow.

Library preparation and indexing

Library-preparation reagents and index components support the relevant libraries and sample identification.

Works with fresh cells, dissociated tissue and nuclei; special sample types and species can be assessed up front.

CHOOSE THE READOUT

Select an RNA or chromatin assay.

RNA studies

Discuss workflows for 3′ gene expression or project-specific targeted RNA studies.

Explore RNA services

Chromatin studies

Investigate accessibility through a defined assay such as ATAC to add a view of cellular regulatory state.

Explore ATAC services

Joint assays and immune repertoires

RNA + ATAC, 5′ gene expression and TCR/BCR-related workflows are under optimization. Project needs and assessment pathways can be discussed.

View project status

RNA and ATAC are separate workflows, measured independently.

Feedback, suggestions or concerns: CEO Direct

Explore cell state, immune and regulatory research