Targeted DNA studies
Start from your target regions to resolve mutation co-occurrence in single cells and rebuild clonal composition and hierarchy.
View DNA services All platformsLink mutations and phenotype
D100 · SERIES · HIGH-THROUGHPUT SINGLE-CELL DNA + PROTEIN
Link mutations and phenotype
in every single cell.
ZYENRA D100 links targeted DNA variants and selected surface-protein signals in the same cell. It reads out which mutations co-occur in each cell and which surface markers each clone carries, giving haematological-cancer, solid-tumour and translational studies clone-level and phenotype-level evidence.
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CONFIGURE YOUR STUDY
Choose measurements around your research question.
DNA + protein studies
Read mutation combinations and surface proteins from the same cell, and compare surface markers and differentiation features across clones.
View multimodal servicesOncology research panels
32 DNA and protein panel designs for haematological and solid tumours. Now recruiting partner laboratories.
Browse the panel catalogue500–20,000
Cells captured per single-chip run
DNA + Protein
Linked readout from the same cell
On-board
Temperature-controlled steps run in the instrument
RVQC®
Real-Time Visual Quality Control System (optional)
HOW IT WORKS
A shared cellular origin.
Two molecular perspectives.
Microfluidic partitioning and cell-barcode association link targeted DNA information and selected surface-protein signals to the same cell during analysis.
One cellular origin
Cellular barcode association
DNA
Targeted genetic variants
Study variant combinations within cells across the selected target regions.
PROTEIN
Selected surface proteins
Use the selected protein markers to examine the surface-protein phenotype of the same cell.
Which variants co-occur?Which protein phenotypes accompany different clones?How does population composition change?
INSTRUMENT & PROCESS
Key steps run inside the instrument.
Droplet encapsulation, droplet fusion and temperature-controlled processing all run inside the instrument, reducing manual transfers. A single chip run captures 500–20,000 cells; libraries are sequenced on a compatible high-throughput platform, and the analysis pipeline links each cell's genotype with its surface-protein signals.
01
Droplet encapsulation & fusion
Cell-containing droplets merge with barcode beads and amplification reagents, giving target DNA and antibody tags a shared cell-of-origin barcode.
02
Temperature control & reactions
A built-in temperature module handles lysis and reaction temperatures on the instrument, without transfer to external equipment.
03
RVQC® System (optional)
With the optional RVQC® Real-Time Visual Quality Control System, real-time visual recognition monitors key droplet states.
WORKFLOW
Workflow | From sample assessment to data analysis
- 01
Assess samples and targets
Define sample conditions, target regions and relevant protein markers.
- 02
Single-cell experiment
D100 and its reagents perform surface-protein labelling, droplet encapsulation, lysis and barcoding.
- 03
Prepare libraries and sequence
Build libraries and generate data on a compatible external high-throughput sequencer.
- 04
Quality control and association analysis
Use cell calling, target coverage and protein background metrics to link each cell's variants and phenotype.
INPUTS & OUTPUTS
What to prepare and how to define deliverables.
Define samples and assay scope
Samples are prepared as high-quality single-cell suspensions, such as bone marrow, peripheral blood, or dissociated tissue and effusions. Before the project starts we assess fit against sample condition, target regions and protein markers, with a pilot run where useful.
Agree the data and analysis scope
Deliverables include raw data, a quality report, targeted variants and selected protein signals, and clone–phenotype association analysis with methods. Quality requirements and acceptance criteria are agreed before the project starts.
TWO WAYS TO WORK
Equip your lab or work with our service team.
Run experiments in your lab
Discuss the D100 instrument, reagents, targeted content and workflow configuration for your lab, including how sequencing and analysis will be arranged.
Discuss instruments and reagentsPlan a research service project
Start with sample assessment, then select targeted DNA, DNA + protein or an assessed custom project and agree the assay scope, quality requirements and deliverables.
Discuss research services View DNA and multimodal service scopeAPPLICATIONS
Tumor heterogeneity and clonal research.
At four points in a study — remission assessment, relapse or resistance, before and after targeted therapy, and when molecular and flow results disagree — D100 aligns genotype and phenotype in the same cell: it resolves where residual clones come from, tracks subclones that expand after treatment and compares target distribution across key clones.
Tumor heterogeneity and clonal researchTarget regions and protein markers are defined by the study design.
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